Nature Communications (Feb 2017)

CRISPR/Cpf1-mediated DNA-free plant genome editing

  • Hyeran Kim,
  • Sang-Tae Kim,
  • Jahee Ryu,
  • Beum-Chang Kang,
  • Jin-Soo Kim,
  • Sang-Gyu Kim

DOI
https://doi.org/10.1038/ncomms14406
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 7

Abstract

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Cpf1 is a type V CRISPR effector protein that has different target and guide RNA requirements compared to Cas9, thus offering an addition tool for precision genome editing. Here Kimet al. show that Cpf1 ribonucleoprotein can be introduced into protoplasts and used for transgene-free gene editing in plants.