Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia

Tianyu Jia; Tianyu Jia; Jingan Rao; Lifang Zou; Lifang Zou; Shanhong Zhao; Shanhong Zhao; Zhihua Yi; Zhihua Yi; Bing Wu; Bing Wu; Lin Li; Lin Li; Huilong Yuan; Huilong Yuan; Liran Shi; Liran Shi; Chunping Zhang; Chunping Zhang; Yun Gao; Yun Gao; Shuangmei Liu; Shuangmei Liu; Hong Xu; Hong Xu; Hui Liu; Hui Liu; Shangdong Liang; Shangdong Liang; Guilin Li; Guilin Li

doi:10.3389/fnins.2017.00755

Frontiers in Neuroscience (Jan 2018)

Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia

Tianyu Jia,
Tianyu Jia,
Jingan Rao,
Lifang Zou,
Lifang Zou,
Shanhong Zhao,
Shanhong Zhao,
Zhihua Yi,
Zhihua Yi,
Bing Wu,
Bing Wu,
Lin Li,
Lin Li,
Huilong Yuan,
Huilong Yuan,
Liran Shi,
Liran Shi,
Chunping Zhang,
Chunping Zhang,
Yun Gao,
Yun Gao,
Shuangmei Liu,
Shuangmei Liu,
Hong Xu,
Hong Xu,
Hui Liu,
Hui Liu,
Shangdong Liang,
Shangdong Liang,
Guilin Li,
Guilin Li

Affiliations

Tianyu Jia: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Tianyu Jia: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Jingan Rao: Second Clinical Department, Medical School, Nanchang University, Nanchang, China
Lifang Zou: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Lifang Zou: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Shanhong Zhao: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Shanhong Zhao: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Zhihua Yi: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Zhihua Yi: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Bing Wu: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Bing Wu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Lin Li: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Lin Li: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Huilong Yuan: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Huilong Yuan: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Liran Shi: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Liran Shi: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Chunping Zhang: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Chunping Zhang: Department of Cell Biology, Medical School, Nanchang University, Nanchang, China
Yun Gao: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Yun Gao: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Shuangmei Liu: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Shuangmei Liu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Hong Xu: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Hong Xu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Hui Liu: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Hui Liu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Shangdong Liang: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Shangdong Liang: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Guilin Li: Department of Physiology, Medical School, Nanchang University, Nanchang, China
Guilin Li: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China

DOI: https://doi.org/10.3389/fnins.2017.00755
Journal volume & issue: Vol. 11

Abstract

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Diabetic peripheral neuropathy results in diabetic neuropathic pain (DNP). Satellite glial cells (SGCs) enwrap the neuronal soma in the dorsal root ganglia (DRG). The purinergic 2 (P2) Y12 receptor is expressed on SGCs in the DRG. SGC activation plays an important role in the pathogenesis of DNP. Curcumin has anti-inflammatory and antioxidant properties. Because curcumin has poor metabolic stability in vivo and low bioavailability, nanoparticle-encapsulated curcumin was used to improve its targeting and bioavailability. In the present study, our aim was to investigate the effects of nanoparticle-encapsulated curcumin on DNP mediated by the P2Y12 receptor on SGCs in the rat DRG. Diabetic peripheral neuropathy increased the expression levels of the P2Y12 receptor on SGCs in the DRG and enhanced mechanical and thermal hyperalgesia in rats with diabetes mellitus (DM). Up-regulation of the P2Y12 receptor in SGCs in the DRG increased the production of pro-inflammatory cytokines. Up-regulation of interleukin-1β (IL-1β) and connexin43 (Cx43) resulted in mechanical and thermal hyperalgesia in rats with DM. The nanoparticle-encapsulated curcumin decreased up-regulated IL-1β and Cx43 expression and reduced levels of phosphorylated-Akt (p-Akt) in the DRG of rats with DM. The up-regulation of P2Y12 on SGCs and the up-regulation of the IL-1β and Cx43 in the DRG indicated the activation of SGCs in the DRG. The nano-curcumin treatment inhibited the activation of SGCs accompanied by its anti-inflammatory effect to decrease the up-regulated CGRP expression in the DRG neurons. Therefore, the nanoparticle-encapsulated curcumin treatment decreased the up-regulation of the P2Y12 receptor on SGCs in the DRG and decreased mechanical and thermal hyperalgesia in rats with DM.

Published in Frontiers in Neuroscience

ISSN: 1662-4548 (Print); 1662-453X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: http://www.frontiersin.org/neuroscience

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