eLife (Dec 2016)
Calcium-mediated actin reset (CaAR) mediates acute cell adaptations
- Pauline Wales,
- Christian E Schuberth,
- Roland Aufschnaiter,
- Johannes Fels,
- Ireth García-Aguilar,
- Annette Janning,
- Christopher P Dlugos,
- Marco Schäfer-Herte,
- Christoph Klingner,
- Mike Wälte,
- Julian Kuhlmann,
- Ekaterina Menis,
- Laura Hockaday Kang,
- Kerstin C Maier,
- Wenya Hou,
- Antonella Russo,
- Henry N Higgs,
- Hermann Pavenstädt,
- Thomas Vogl,
- Johannes Roth,
- Britta Qualmann,
- Michael M Kessels,
- Dietmar E Martin,
- Bela Mulder,
- Roland Wedlich-Söldner
Affiliations
- Pauline Wales
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Christian E Schuberth
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Roland Aufschnaiter
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Johannes Fels
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Ireth García-Aguilar
- ORCiD
- Theory of Biological Matter, FOM Institute AMOLF, Amsterdam, Netherlands
- Annette Janning
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Christopher P Dlugos
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany; Medical Clinic D, University Clinic of Muenster, Muenster, Germany
- Marco Schäfer-Herte
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Christoph Klingner
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany; AG Molecular Mechanotransduction, Max Planck Institute of Biochemistry, Munich, Germany
- Mike Wälte
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Julian Kuhlmann
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Ekaterina Menis
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Laura Hockaday Kang
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- Kerstin C Maier
- Department of Biochemistry, University of Munich, Munich, Germany
- Wenya Hou
- Institute of Biochemistry I, Friedrich Schiller University Jena, Jena, Germany
- Antonella Russo
- Institute of Immunology, University of Münster, Münster, Germany
- Henry N Higgs
- Department of Biochemistry, Dartmouth Medical School, Hanover, United States
- Hermann Pavenstädt
- Medical Clinic D, University Clinic of Muenster, Muenster, Germany
- Thomas Vogl
- Institute of Immunology, University of Münster, Münster, Germany
- Johannes Roth
- Institute of Immunology, University of Münster, Münster, Germany
- Britta Qualmann
- Institute of Biochemistry I, Friedrich Schiller University Jena, Jena, Germany
- Michael M Kessels
- Institute of Biochemistry I, Friedrich Schiller University Jena, Jena, Germany
- Dietmar E Martin
- Department of Biochemistry, University of Munich, Munich, Germany
- Bela Mulder
- Theory of Biological Matter, FOM Institute AMOLF, Amsterdam, Netherlands
- Roland Wedlich-Söldner
- ORCiD
- Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany; Cells-In-Motion Cluster of Excellence (EXC1003 – CiM), University of Münster, Muenster, Germany
- DOI
- https://doi.org/10.7554/eLife.19850
- Journal volume & issue
-
Vol. 5
Abstract
Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased intracellular calcium levels. Within seconds of calcium influx, the formin INF2 stimulates filament polymerization at the endoplasmic reticulum (ER), while cortical actin is disassembled. The reaction is then reversed within a few minutes. This Calcium-mediated actin reset (CaAR) occurs in a wide range of mammalian cell types and in response to many physiological cues. CaAR leads to transient immobilization of organelles, drives reorganization of actin during cell cortex repair, cell spreading and wound healing, and induces long-lasting changes in gene expression. Our findings suggest that CaAR acts as fundamental facilitator of cellular adaptations in response to acute signals and stress.
Keywords
