Dataset of Nile Red Fluorescence Readings with Different Yeast Strains, Solvents, and Incubation Times
Mauricio Ramirez-Castrillon,
Victoria P. Jaramillo-Garcia,
Helio Lopes Barros,
João A. Pêgas Henriques,
Valter Stefani,
Patricia Valente
Affiliations
Mauricio Ramirez-Castrillon
Research Group in Mycology (GIM/CICBA), Universidad Santiago de Cali, Calle 5 No. 62-00, Santiago de Cali, Colombia
Victoria P. Jaramillo-Garcia
Graduate Program in Cell and Molecular Biology, Biotechnology Center, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500 Prédios 43421/43431 - Setor IV - Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil
Helio Lopes Barros
New Organic Materials and Forensic Chemistry Laboratory (LNMO-QF), Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil
João A. Pêgas Henriques
Graduate Program in Cell and Molecular Biology, Biotechnology Center, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500 Prédios 43421/43431 - Setor IV - Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil
Valter Stefani
New Organic Materials and Forensic Chemistry Laboratory (LNMO-QF), Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil
Patricia Valente
Department of Microbiology, Immunology and Parasitology, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS CEP 90050-170, Brazil
We used Nile red to estimate lipid content in oleaginous yeasts using a high-throughput approach. We measured the fluorescence intensity of Nile red using different solvents, yeast strains, and incubation times in optimized excitation/emission wavelengths. The data show the relative fluorescence units (RFU) for Nile red excitation, using 1× PBS, 1× PBS and 5% v/v isopropyl alcohol, 50% v/v glycerol, culture medium A-gly broth, and A-gly broth supplemented with 5% v/v DMSO. In addition, we showed the RFU for the Nile red dye for different oleaginous and non-oleaginous yeast strains, such as Meyerozyma guilliermondii BI281A, Yarrowia lipolytica QU21 and Saccharomyces cerevisiae MRC164. Other measurements of lipid accumulation kinetics were shown for the above and additional yeast strains. These datasets provide the guidelines to obtain the optimal solvent system and the minimal interaction time for the Nile red dye to enter in the cells and obtain a stable readout.
