Ag-Contained Superabsorbent Curdlan–Chitosan Foams for Healing Wounds in a Type-2 Diabetic Mice Model
Elizaveta S. Permyakova,
Anton S. Konopatsky,
Konstantin I. Ershov,
Ksenia I. Bakhareva,
Natalya A. Sitnikova,
Dmitry V. Shtansky,
Anastasiya O. Solovieva,
Anton M. Manakhov
Affiliations
Elizaveta S. Permyakova
Laboratory of Inorganic Nanomaterials, National University of Science and Technology “MISiS”, Leninsky Prospekt 4, 119049 Moscow, Russia
Anton S. Konopatsky
Laboratory of Inorganic Nanomaterials, National University of Science and Technology “MISiS”, Leninsky Prospekt 4, 119049 Moscow, Russia
Konstantin I. Ershov
Laboratory of Pharmacological Active Compounds, Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova Str., 630060 Novosibirsk, Russia
Ksenia I. Bakhareva
Laboratory of Pharmacological Active Compounds, Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova Str., 630060 Novosibirsk, Russia
Natalya A. Sitnikova
Laboratory of Pharmacological Active Compounds, Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova Str., 630060 Novosibirsk, Russia
Dmitry V. Shtansky
Laboratory of Inorganic Nanomaterials, National University of Science and Technology “MISiS”, Leninsky Prospekt 4, 119049 Moscow, Russia
Anastasiya O. Solovieva
Laboratory of Pharmacological Active Compounds, Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova Str., 630060 Novosibirsk, Russia
Anton M. Manakhov
Laboratory of Pharmacological Active Compounds, Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova Str., 630060 Novosibirsk, Russia
This study focused on the synthesis and characterization of pure curdlan–chitosan foams (CUR/CS), as well as foams containing Ag nanoparticles (CUR/CS/Ag), and their effect on the skin repair of diabetic mice (II type). The layer of antibacterial superabsorbent foam provides good oxygenation, prevents bacterial infection, and absorbs exudate, forming a soft gel (moist environment). These foams were prepared from a mixture of hydrolyzed curdlan and chitosan by lyophilization. To enhance the antibacterial properties, an AgNO3 solution was added to the curdlan/chitosan mixture during the polymerization and was then reduced by UV irradiation. The membranes were further investigated for their structure and composition using optical microscopy, scanning electron microscopy, energy-dispersive spectroscopy, FT-IR spectroscopy, and XPS analysis and modeling. In vivo tests demonstrated that CUR/CS/Ag significantly boosted the regeneration process compared with pure CUR/CS and the untreated control.
