Informatively of histological study by method of mapping ellipticity of polarization of microscopic myocardial images

Abstract

Purpose of the study. To investigate the informational value of the histological examination of the human myocardium in case of sudden cardiac death and the possibility of a method of mapping ellipticity of polarization of microscopic images of the myocardium in the diagnosis and objective differentiation of acute coronary insufficiency (ACI) and chronic coronary heart disease (CIHD). Materials and methods. Samples of human myocardium from 89 people who died as a result of ACI and CIHD were investigated, using traditional histological and laser polarization techniques, with a standard-scheme laser polarimeter. Subsequently, statistical processing of the obtained results was conducted. Results. The results obtained show that the values of the statistical moments of the 3rd and 4th order, which characterize the coordinate distributions of the ellipticity of polarization of microscopic images of myocardial samples on the scale of optical magnification ×56, were the most informative for differential diagnosis of ACI and CIHD. Accuracy of ACI and CIHD detection by mapping the ellipticity of polarization of myocardial microscopic images at an optical magnification of ×56 reaches ~ 97%, as opposed to the traditional histological examination ~ 81%. Conclusion. On the basis of the obtained data, we confirm the diagnostic efficiency and accuracy of the method of mapping the values of ellipticity polarization of microscopic images of histological sections of the myocardium in the problem of diagnosis and objective differentiation of ACI and CIHD.

Keywords

• laser polarimetry
• histology
• myocardium
• sudden cardiac death