In vitro inhibitory potential of two synthesized fatty amides derivatives against some microbial pathogens

Abstract

In the present study, two amide derivatives with long chains; N-cyclohexyloctamide 1 and NPhenyldodecanamide (dodecanilide) 2 were synthesized. They were tested in vitro for a wide array of antimicrobial activities against; two Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus), four Gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella enteric), and three fungi (Aspergillus carbonarius, Mucor ramannianus, Candida albicans), to evaluate their potential use as antimicrobial agents. Zones of inhibition of these compounds were determined by disc diffusion assay. Results revealed high antimicrobial activities of the two amides. The new fatty N-cyclohexyloctamide 1 showed significant antimicrobial activity against S. aureus and A. carbonarius, and was more active against C. albicans than synthesized fatty anilide 2, as it caused higher zones of inhibition. On the other hand, the fatty anilide 2 demonstrated high antibacterial activity against P. aeruginosa, and was more active against S. enteric than substituted fatty amide 1. On the basis of these obtained results, it could be assumed that fatty amide derivatives may be used as components of disinfectants, and may be possible sources of new drugs effective against various infectious diseases.

Keywords

• amide derivatives
• synthesis
• antimicrobial activity
• disk diffusion assay