Pyramiding of multiple genes generates rapeseed introgression lines with clubroot and herbicide resistance, high oleic acid content, and early maturity
Zhaoyang Wang,
Fucai Wang,
Zihan Yu,
Xiaorui Shi,
Xianming Zhou,
Pengfei Wang,
Yixian Song,
Dengfeng Hong,
Guangsheng Yang
Affiliations
Zhaoyang Wang
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Fucai Wang
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Zihan Yu
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Xiaorui Shi
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Xianming Zhou
Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya 572025, Hainan, China; College of Tropical Crops Hainan University, Hainan University, Haikou 570288, Hainan, China
Pengfei Wang
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Yixian Song
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Dengfeng Hong
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Hubei Hongshan Laboratory, Wuhan 430070, Hubei, China
Clubroot and herbicide resistance, high oleic acid (OA) content, and early maturity are targets of rapeseed (Brassica napus L.) breeding. The objective of this study was to develop new male-fertility restorer lines by pyramiding favorable genes to improve these traits simultaneously. Seven elite alleles for the four traits were introduced into the restorer line 621R by speed breeding with marker-assisted and phenotypic selection. Six introgression lines (ILs) were developed with four- to seven-gene combinations and crossed with two elite parents to develop hybrids. All ILs and their corresponding hybrids displayed high resistance to both clubroot pathotype 4 and sulfonylurea herbicides. Three ILs and their hybrids showed large increases in OA contents and four showed earlier maturity. These new ILs may be useful in rapeseed hybrid breeding for the target traits.
