Next-generation lung cancer pathology: Development and validation of diagnostic and prognostic algorithms

Carina Kludt; Yuan Wang; Waleed Ahmad; Andrey Bychkov; Junya Fukuoka; Nadine Gaisa; Mark Kühnel; Danny Jonigk; Alexey Pryalukhin; Fabian Mairinger; Franziska Klein; Anne Maria Schultheis; Alexander Seper; Wolfgang Hulla; Johannes Brägelmann; Sebastian Michels; Sebastian Klein; Alexander Quaas; Reinhard Büttner; Yuri Tolkach

Cell Reports Medicine (Sep 2024)

Next-generation lung cancer pathology: Development and validation of diagnostic and prognostic algorithms

Carina Kludt,
Yuan Wang,
Waleed Ahmad,
Andrey Bychkov,
Junya Fukuoka,
Nadine Gaisa,
Mark Kühnel,
Danny Jonigk,
Alexey Pryalukhin,
Fabian Mairinger,
Franziska Klein,
Anne Maria Schultheis,
Alexander Seper,
Wolfgang Hulla,
Johannes Brägelmann,
Sebastian Michels,
Sebastian Klein,
Alexander Quaas,
Reinhard Büttner,
Yuri Tolkach

Affiliations

Carina Kludt: Institute of Pathology, University Hospital Cologne, 50937 Cologne, Germany
Yuan Wang: Institute of Pathology, University Hospital Cologne, 50937 Cologne, Germany
Waleed Ahmad: Institute of Pathology, University Hospital Cologne, 50937 Cologne, Germany
Andrey Bychkov: Department of Pathology, Kameda Medical Center, Kamogawa 296-0041, Japan; Department of Pathology Informatics, Nagasaki University, Nagasaki 852-8131, Japan
Junya Fukuoka: Department of Pathology, Kameda Medical Center, Kamogawa 296-0041, Japan; Department of Pathology Informatics, Nagasaki University, Nagasaki 852-8131, Japan
Nadine Gaisa: Institute of Pathology, University Hospital Aachen, 52074 Aachen, Germany; Institute of Pathology, University Hospital Ulm, 89081 Ulm, Germany
Mark Kühnel: Institute of Pathology, University Hospital Aachen, 52074 Aachen, Germany
Danny Jonigk: Institute of Pathology, University Hospital Aachen, 52074 Aachen, Germany; German Center for Lung Research, DZL, BREATH, 30625 Hanover, Germany
Alexey Pryalukhin: Institute of Clinical Pathology and Molecular Pathology, Wiener Neustadt State Hospital, 2700 Wiener Neustadt, Austria
Fabian Mairinger: Institute of Pathology, University Hospital Essen, 45147 Essen, Germany
Franziska Klein: Institute of Pathology, University Hospital Cologne, 50937 Cologne, Germany
Anne Maria Schultheis: Institute of Pathology, University Hospital Cologne, 50937 Cologne, Germany; Medical Faculty University of Cologne, 50937 Cologne, Germany
Alexander Seper: Institute of Clinical Pathology and Molecular Pathology, Wiener Neustadt State Hospital, 2700 Wiener Neustadt, Austria; Danube Private University, 3500 Krems an der Donau, Austria
Wolfgang Hulla: Institute of Clinical Pathology and Molecular Pathology, Wiener Neustadt State Hospital, 2700 Wiener Neustadt, Austria
Johannes Brägelmann: University of Cologne, Faculty of Medicine and University Hospital Cologne, Department of Translational Genomics, 50937 Cologne, Germany; Mildred Scheel School of Oncology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50937 Cologne, Germany; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Molecular Medicine Cologne, 50937 Cologne, Germany
Sebastian Michels: University of Cologne, Faculty of Medicine and University Hospital of Colone, Lung Cancer Group Cologne, Department I for Internal Medicine and Center for Integrated Oncology Aachen Bonn Cologne Dusseldorf, 50937 Cologne, Germany
Sebastian Klein: Institute of Pathology, University Hospital Cologne, 50937 Cologne, Germany; Medical Faculty University of Cologne, 50937 Cologne, Germany
Alexander Quaas: Institute of Pathology, University Hospital Cologne, 50937 Cologne, Germany; Medical Faculty University of Cologne, 50937 Cologne, Germany
Reinhard Büttner: Institute of Pathology, University Hospital Cologne, 50937 Cologne, Germany; Medical Faculty University of Cologne, 50937 Cologne, Germany; Corresponding author
Yuri Tolkach: Institute of Pathology, University Hospital Cologne, 50937 Cologne, Germany; Medical Faculty University of Cologne, 50937 Cologne, Germany; Corresponding author

Journal volume & issue: Vol. 5, no. 9
p. 101697

Abstract

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Summary: Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors. In this study, we develop a clinically useful computational pathology platform for NSCLC that can be a foundation for multiple downstream applications and provide immediate value for patient care optimization and individualization. We train the primary multi-class tissue segmentation algorithm on a substantial, high-quality, manually annotated dataset of whole-slide images with lung adenocarcinoma and squamous cell carcinomas. We investigate two downstream applications. NSCLC subtyping algorithm is trained and validated using a large, multi-institutional (n = 6), multi-scanner (n = 5), international cohort of NSCLC cases (slides/patients 4,097/1,527). Moreover, we develop four AI-derived, fully explainable, quantitative, prognostic parameters (based on tertiary lymphoid structure and necrosis assessment) and validate them for different clinical endpoints. The computational platform enables the high-precision, quantitative analysis of H&E-stained slides. The developed prognostic parameters facilitate robust and independent risk stratification of patients with NSCLC.

Published in Cell Reports Medicine

ISSN: 2666-3791 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General)
Website: https://www.cell.com/cell-reports-medicine

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Abstract

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