Preventing erosion of X-chromosome inactivation in human embryonic stem cells

Marissa Cloutier; Surinder Kumar; Emily Buttigieg; Laura Keller; Brandon Lee; Aaron Williams; Sandra Mojica-Perez; Indri Erliandri; Andre Monteiro Da Rocha; Kenneth Cadigan; Gary D. Smith; Sundeep Kalantry

doi:10.1038/s41467-022-30259-x

Nature Communications (May 2022)

Preventing erosion of X-chromosome inactivation in human embryonic stem cells

Marissa Cloutier,
Surinder Kumar,
Emily Buttigieg,
Laura Keller,
Brandon Lee,
Aaron Williams,
Sandra Mojica-Perez,
Indri Erliandri,
Andre Monteiro Da Rocha,
Kenneth Cadigan,
Gary D. Smith,
Sundeep Kalantry

Affiliations

Marissa Cloutier: Department of Human Genetics, University of Michigan Medical School
Surinder Kumar: Department of Human Genetics, University of Michigan Medical School
Emily Buttigieg: Department of Human Genetics, University of Michigan Medical School
Laura Keller: Department of Molecular & Integrative Physiology, University of Michigan Medical School
Brandon Lee: Department of Human Genetics, University of Michigan Medical School
Aaron Williams: Department of Human Genetics, University of Michigan Medical School
Sandra Mojica-Perez: Department of Molecular & Integrative Physiology, University of Michigan Medical School
Indri Erliandri: Department of Molecular & Integrative Physiology, University of Michigan Medical School
Andre Monteiro Da Rocha: Department of Molecular & Integrative Physiology, University of Michigan Medical School
Kenneth Cadigan: Department of Molecular, Cellular, and Developmental Biology, University of Michigan Medical School
Gary D. Smith: Department of Molecular & Integrative Physiology, University of Michigan Medical School
Sundeep Kalantry: Department of Human Genetics, University of Michigan Medical School

DOI: https://doi.org/10.1038/s41467-022-30259-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 18

Abstract

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Cloutier et al. discover that human embryonic stem cells (hESCs) cultured with media containing inhibitors of GSK3 proteins undergo erosion of X-chromosome inactivation, which equalizes X-linked gene expression between females and males. The findings inform the faithful culture of hESCs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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