Multifeatured guar gum armed drug delivery system for the delivery of ofloxacin drug to treat ophthalmic dieases

Abstract

Nanotargeting delivery system is recently much more attention for delivering drugs and bioactive compounds to treating ocular diseases. Here, the Guar gum (GG) armed drug delivery system with functionalization of poly-caprolactone (PCL), Retinol (RA), Biotinylated-Glutathione (B-GHS), carried out by the condensation reactions. The functional changes and crystallinity of the prepared micelle system were characterized by the FTIR and XRD techniques. The SEM observation represents the morphology of micelle GG-g-PCL-RA-B-GHS; it shows sphere-like morphology, and it was correlated with TEM results. The unloaded and drug-loaded micelle’s average spherical diameter was found as ~4.5 and ~6.8 nm, respectively, by the ImageJ software analysis. The carrier systems’ surface charge was found as a negative value from zeta potential analysis. From in-vitro drug release studies, the drug release in a tear fluid environment (PBS, pH 7.4) was observed 88.0% of OFL in 8 h, and the drug release rate renewed in a controlled manner leads to the requirement for prolonged drug delivery. The antibacterial activity, the incubation of OFL loaded GG-g-PCL-RA-B-GHS micelle groups, found an excellent antibacterial activity against both gram-negative Salmonella Typhi and gram-positive Bacillus cereus. Since the novel OFL loaded GG-g-PCL-RA-B-GHS maintaining a prominent role in the delivery of OFL and good antibacterial activity, it could be offered as a promising strategy for ocular drug delivery.

Keywords

• Biotin
• Guar gum
• Ofloxacin
• Opthalmatic treatment
• Polycaprolactone
• Targeted delivery