Caracterización de cepas nativas de Clostridium spp por secuenciación parcial del gen ribosomal 16s rARN

Abstract

Solventogenic and acidogenic Clostridium has been characterized by biochemical, physiological and molecular techniques. These microorganisms have been classified into four groups but these may not necessarily be the only four exclusive groups; in fact, many of these microorganisms might not belong to any of these selected groups. The main goal was to classify 13 higher solvent- producing Clostridia strains through molecular biology techniques; strains were isolated by our Group. Sequence analysis was calculated by p and the dendogram was constructed by neighbor-joining. The results obtained strongly suggest the idea of a high similarity between the 13 native strains in the study and the fact that they had no relationship to any of the 4 groups established by Keis et al (1995). It was found that Clostridium butyricum strain is the most related specie to the thirteen Colombian strains.

Keywords

• Clostridium spp
• cepas nativas
• cepas productoras de solvents
• 16S rARN
• caracterización molecula