Fishing for newly synthesized proteins with phosphonate-handles

Fleur Kleinpenning; Barbara Steigenberger; Wei Wu; Albert J. R. Heck

doi:10.1038/s41467-020-17010-0

Nature Communications (Jun 2020)

Fishing for newly synthesized proteins with phosphonate-handles

Fleur Kleinpenning,
Barbara Steigenberger,
Wei Wu,
Albert J. R. Heck

Affiliations

Fleur Kleinpenning: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
Barbara Steigenberger: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
Wei Wu: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
Albert J. R. Heck: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University

DOI: https://doi.org/10.1038/s41467-020-17010-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

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Here the authors describe PhosID, an enrichment strategy using phosphonate-handles, that combines click chemistry and IMAC-based phospho-enrichment for quantitative proteomics analysis of newly synthesized proteins.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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