Self-assembled peptide-paclitaxel nanoparticles for enhancing therapeutic efficacy in colorectal cancer

Lidan Hou; Lidan Hou; Lidan Hou; Ting Zhong; Ting Zhong; Ting Zhong; Peng Cheng; Peng Cheng; Bohan Long; Bohan Long; Bohan Long; Leilei Shi; Xiangjun Meng; Xiangjun Meng; Xiangjun Meng; Han Yao; Han Yao; Han Yao

doi:10.3389/fbioe.2022.938662

Frontiers in Bioengineering and Biotechnology (Sep 2022)

Self-assembled peptide-paclitaxel nanoparticles for enhancing therapeutic efficacy in colorectal cancer

Lidan Hou,
Lidan Hou,
Lidan Hou,
Ting Zhong,
Ting Zhong,
Ting Zhong,
Peng Cheng,
Peng Cheng,
Bohan Long,
Bohan Long,
Bohan Long,
Leilei Shi,
Xiangjun Meng,
Xiangjun Meng,
Xiangjun Meng,
Han Yao,
Han Yao,
Han Yao

Affiliations

Lidan Hou: Department of Gastroenterology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Lidan Hou: The Digestive Disease Research and Clinical Transformation Center, Shanghai Jiao Tong University, Shanghai, China
Lidan Hou: Shanghai Key Laboratory of Gut Microecology and Associated Diseases, Shanghai, China
Ting Zhong: Department of Gastroenterology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Ting Zhong: The Digestive Disease Research and Clinical Transformation Center, Shanghai Jiao Tong University, Shanghai, China
Ting Zhong: Shanghai Key Laboratory of Gut Microecology and Associated Diseases, Shanghai, China
Peng Cheng: Department of Gastroenterology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Peng Cheng: Department of Gastroenterology, Hainan West Central Hospital, Hainan, China
Bohan Long: Department of Gastroenterology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Bohan Long: The Digestive Disease Research and Clinical Transformation Center, Shanghai Jiao Tong University, Shanghai, China
Bohan Long: Shanghai Key Laboratory of Gut Microecology and Associated Diseases, Shanghai, China
Leilei Shi: The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
Xiangjun Meng: Department of Gastroenterology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Xiangjun Meng: The Digestive Disease Research and Clinical Transformation Center, Shanghai Jiao Tong University, Shanghai, China
Xiangjun Meng: Shanghai Key Laboratory of Gut Microecology and Associated Diseases, Shanghai, China
Han Yao: Department of Gastroenterology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Han Yao: The Digestive Disease Research and Clinical Transformation Center, Shanghai Jiao Tong University, Shanghai, China
Han Yao: Shanghai Key Laboratory of Gut Microecology and Associated Diseases, Shanghai, China

DOI: https://doi.org/10.3389/fbioe.2022.938662
Journal volume & issue: Vol. 10

Abstract

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Chemotherapy is one of the main treatments for colorectal cancer, but systemic toxicity severely limits its clinical use. Packaging hydrophobic chemotherapeutic drugs in targeted nanoparticles greatly improve their efficacy and reduce side effects. We previously identified a novel colorectal cancer specific binding peptide P-LPK (LPKTVSSDMSLN) from phage display peptide library. Here we designed a self-assembled paclitaxel (PTX)-loaded nanoparticle (LPK-PTX NPs). LPK-PTX NPs displayed a superior intracellular internalization and improved tumor cytotoxicity in vitro. Cy5.5-labeled LPK-PTX NPs showed much higher tumor accumulation in colorectal cancer-bearing mice. Furthermore, LPK-PTX NPs exhibit enhanced antitumor activity and decreased systemic toxicity in colorectal cancer patient-derived xenografts (PDX) model. The excellent in vitro and in vivo antitumor efficacy proves the improved targeting drug delivery, suggesting that peptide P-LPK has potential to provide a novel approach for enhanced drug delivery with negligible systemic toxicity.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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