OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth
Yaqian Huang,
Xiaodi Gong,
Hui Shi,
Peiyi Wang,
Yundong Yuan,
Cuilian Kong,
Jie Zhou,
Dianxing Wu,
Yan Liang,
Yonghong Wang,
Jing Wang
Affiliations
Yaqian Huang
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100039, China
Xiaodi Gong
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100039, China
Hui Shi
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China
Peiyi Wang
College of Life Sciences, Shandong Agriculture University, Tai’an, Shandong 271018, China
Yundong Yuan
College of Life Sciences, Shandong Agriculture University, Tai’an, Shandong 271018, China
Cuilian Kong
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China
Jie Zhou
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Dianxing Wu
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Yan Liang
College of Life Sciences, Shandong Agriculture University, Tai’an, Shandong 271018, China
Yonghong Wang
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100039, China; College of Life Sciences, Shandong Agriculture University, Tai’an, Shandong 271018, China; Corresponding author
Jing Wang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; College of Agronomy, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Corresponding author
Summary: Keeping nucleotide-binding leucine-rich repeat (NLR) protein at appropriate levels is critical for plant survival. Huntingtin Yeast Partner K (OsHYPK) was previously identified as a positive regulator of N-terminal acetyltransferase A (NatA) activity in rice. Here, we find that oshypk shows enhanced resistance to Magnaporthe oryzae (M. oryzae). Through screening for suppressors of oshypk (soh), we identify suppressor soh74, which contains a mutation in RESISTANCE TO P. SYRINGAE PV MACULICOLA1 (RPM1)-like NLR protein (RPM1-L1) and exhibits compromised resistance to M. oryzae. Mechanistically, declined N-terminal acetylation (NTA) degree in oshypk leads to protein accumulation of RPM1-L1, contributing to enhanced disease resistance. To restrict RPM1-L1 accumulation, OsHYPK is expressed at high levels under normal conditions. However, pathogen infection reduces OsHYPK level to release the inhibition on RPM1-L1, leading to immune activation. This study reveals a vital pathway in which OsHYPK/NatA-mediated NTA rapidly fine-tunes NLR-mediated immune response.
