Ciência Rural (Dec 1998)
Enzimas de função hepática na aflatoxicose aguda experimental em frangos de corte Hepatic enzimes function in experimental acute enzimes aflatoxicosis in broilers
Abstract
Com o objetivo de avaliar a função hepática de aves experimentalmente intoxicadas por aflatoxina, com e sem uso de bentonita sódica, foram utilizados 40 (quarenta) frangos de corte, machos, linhagem Ross, divididos em 4 (quatro) grupos de 10 (dez.) animais, sendo que cada grupo foi submetido a um tratamento: T1 - controle (ração sem aflatoxina ou bentonita), T2 - ração com 5ppm de aflatoxina, T3 - ração com 5ppm de aflatoxina e 0,5% de bentonita sódica e T4 - ração com 0,5% de bentonita sódica. Todos estes tratamentos foram aplicados do 1° ao 42 ° dia de vida das aves. Aos 21, 35 e 42 dias de idade, foram analisados os níveis séricos das enzimas aspartato aminotransferase (AST), alanina aminotransferase (ALT), lactato desidrogenase (LDH) e gama glutamiltransferase (GGT), A análise da variância mostrou que houve interação entre os tratamentos e datas de colheita de material, para as seguintes variáveis: AST, LDH e GGT. Para estas, foi aplicado o teste de Tukey, comparando-se as médias de cada tratamento em cada data. Observou-se que as enzimas AST, ALT e GGT não apresentaram diferenças significativas entre tratamentos, porém, nos tratamentos l e 2, a AST apresentou um aumento linear (pThe aim ofthis study is to evaluate ïhe hepatic function of experimentally intoxicated broilers by aflatoxin with and without sodium bentonite. Forty Ross mole broilers, were used divided into 4 groups of 10 birds, and such groups have been submitted to the following treatments: T1- control (feed without aflatoxin or sodium bentonite), T2- feed containing 5ppm of aflatoxin, T3- feed containing 5ppm of aflatoxin and 0.5% of sodium bentonite and T4- feed containing 0.5% of sodium bentonite. Ali these treatments have been appiied from the l st to the 42nd day of lif e. On the days 21, 35 and 42, the serum leveis of the enzimes aspartate aminotransferase (AST), alanino aminotransferase (ALT), lactate dehydrogenase (LDH) and gamma glutamiltransferase (GGT). The analysis of variance showed an interaction between treatments and time of blood sampling for the following variables: AST, LDH and GGT. Tukey test was used to compare significant averages among treatments. It was observed that the enzimes AST, ALT and GGT have not shown significam diferences among treatments. However, in treatment 2, AST showed a linear increase (p < 0.01) along the experiment, more pronounced in treatment 2. The ALT had no variation in any treatment during the total period. The GGT enzyme in treatments 2 and 3 had a linear increase (p < 0.05) for ali periods. The LDH leveis, at the 21st day, was higher for treatments 2 and 3 compared to treatments l and 4. According to the results, it can be concluded that is possible to detect the hepatotoxicity of aflatoxin in through monitoring of enzimes AST and GGT leveis, in the serum of birds between 21 and 42 days of age, and by evaluation ofLDH at 21 days.
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