A highly sensitive microfluidic biosensor for rapid and accurate detection of Salmonella in raw chicken products

Abstract

This paper presents an investigation of a fluidic-based impedance biosensor for rapid and accurate detection of Salmonella Typhimurium in raw chicken carcass rinsate. The biosensor is engineered with multiple distinct regions that concentrates Salmonella antigens to a detectable level, subsequently trapping the concentrated Salmonella samples on top of the detection interdigitated electrode array coated with a specific Salmonella antibody, maximizing the number of captured antigens. Detection is achieved through the antibody-antigen binding process, where binding events changes impedance values, providing a reliable method for identifying and quantifying Salmonella. The biosensor demonstrated a low limit of detection (LOD) of 1–2 cells/ml within 40–50 min. The findings demonstrated that the biosensor distinguishes low concentrations of live Salmonella cells, even in the presence of high concentrations of dead Salmonella cells, and non-specific binding pathogens viz., Listeria monocytogenes and E. coli O157:H7.

Keywords

• Microfluidic
• Biosensor
• Dielectrophoresis
• MEMS
• Impedance
• Salmonella