The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster

Xue Qiao; Xue Qiao; Xue Qiao; Yanan Zong; Yanan Zong; Yanan Zong; Zhaoqun Liu; Zhaoqun Liu; Zhaoqun Liu; Zhaojun Wu; Zhaojun Wu; Zhaojun Wu; Yuanmei Li; Yuanmei Li; Yuanmei Li; Lingling Wang; Lingling Wang; Lingling Wang; Lingling Wang; Lingling Wang; Linsheng Song; Linsheng Song; Linsheng Song; Linsheng Song; Linsheng Song

doi:10.3389/fimmu.2021.689783

Frontiers in Immunology (Jun 2021)

The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster

Xue Qiao,
Xue Qiao,
Xue Qiao,
Yanan Zong,
Yanan Zong,
Yanan Zong,
Zhaoqun Liu,
Zhaoqun Liu,
Zhaoqun Liu,
Zhaojun Wu,
Zhaojun Wu,
Zhaojun Wu,
Yuanmei Li,
Yuanmei Li,
Yuanmei Li,
Lingling Wang,
Lingling Wang,
Lingling Wang,
Lingling Wang,
Lingling Wang,
Linsheng Song,
Linsheng Song,
Linsheng Song,
Linsheng Song,
Linsheng Song

Affiliations

Xue Qiao: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Xue Qiao: Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Xue Qiao: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Yanan Zong: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Yanan Zong: Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Yanan Zong: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Zhaoqun Liu: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Zhaoqun Liu: Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Zhaoqun Liu: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Zhaojun Wu: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Zhaojun Wu: Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Zhaojun Wu: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Yuanmei Li: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Yuanmei Li: Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Yuanmei Li: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Lingling Wang: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Lingling Wang: Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Lingling Wang: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Lingling Wang: Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai, China
Lingling Wang: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China
Linsheng Song: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Linsheng Song: Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Linsheng Song: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Linsheng Song: Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai, China
Linsheng Song: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China

DOI: https://doi.org/10.3389/fimmu.2021.689783
Journal volume & issue: Vol. 12

Abstract

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Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the CgIFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After CgIFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes (CgISGs) was significantly inhibited. Both cyclic GMP-AMP synthase (CgcGAS) and stimulator of interferon gene (CgSTING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of CgIFNLP and interferon regulatory factors (CgIRF1/8) and the nuclear translocation of CgIRF8 were all suppressed in CgcGAS-RNAi or CgSTING-RNAi oysters after Poly (I:C) stimulation. The expression level of CgSTING and TANK binding kinase1 (CgTBK1) did not decrease in CgcGAS-RNAi oysters. After CgSTING was knocked down, the high expression of CgTBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING–TBK1–IRFs regulatory axis in mollusks, which was different from the classic cGAS–STING–TBK1 signal pathway in mammals.

Published in Frontiers in Immunology

ISSN: 1664-3224 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: http://journal.frontiersin.org/journal/immunology

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