Nature Communications (Nov 2017)

Characterizing a thermostable Cas9 for bacterial genome editing and silencing

  • Ioannis Mougiakos,
  • Prarthana Mohanraju,
  • Elleke F. Bosma,
  • Valentijn Vrouwe,
  • Max Finger Bou,
  • Mihris I. S. Naduthodi,
  • Alex Gussak,
  • Rudolf B. L. Brinkman,
  • Richard van Kranenburg,
  • John van der Oost

DOI
https://doi.org/10.1038/s41467-017-01591-4
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 11

Abstract

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CRISPR-Cas9 genome engineering tools have found wide application in a range of species, however they are unsuitable for applications at elevated temperatures. Here the authors characterise ThermoCas9 from which is functional from 20°C to 70°C.