Motriz: Revista de Educacao Fisica (Nov 2018)

Effects of different frequencies of physical training on electron transport chain and oxidative damage in healthy mice

  • Luciano A. Silva,
  • Camila B. Tromm,
  • Ramiro Doyenart,
  • Anand Thirupathi,
  • Paulo Cesar Loock Silveira,
  • Ricardo Aurinho Pinho

DOI
https://doi.org/10.1590/s1980-6574201800040008
Journal volume & issue
Vol. 24, no. 4

Abstract

Read online

Abstract Aims: The present study investigated the effect of different frequencies (three and five times a week) on electron transport chain and oxidative stress after 8 weeks of run training. Methods: Eighteen male mice (CF1, 30-35g) were distributed into the following groups (n=6): untrained (UT); trained three-time per week (T3) and trained five- time per week (T5). All training sessions were at the same intensity and duration (45min/day) in a treadmill for small animals. Forty-eight hours after the last training session, the animals were killed by decapitation and quadriceps (red portion) was removed and stored at -70ºC. Succinate dehydrogenase (SDH), complexes I, II, II-III, IV and hydroperoxides were measured. Results: Training sessions for five times per week were more effective in increasing the mitochondrial respiratory chain enzyme activities (SDH, complexes I, II, II-III, IV) as well as in decreasing the formation hydroperoxides than sessions performed for three times training per week (p<0.05). Conclusion: Our findings clearly showed that a higher the frequency of training session promotes a greater activity of the electron transport chain and consequently reduces the oxidative stress in healthy animals.

Keywords