Structural Dynamics (Nov 2019)
Evaluation of serial crystallographic structure determination within megahertz pulse trains
- Oleksandr Yefanov,
- Dominik Oberthür,
- Richard Bean,
- Max O. Wiedorn,
- Juraj Knoska,
- Gisel Pena,
- Salah Awel,
- Lars Gumprecht,
- Martin Domaracky,
- Iosifina Sarrou,
- P. Lourdu Xavier,
- Markus Metz,
- Saša Bajt,
- Valerio Mariani,
- Yaroslav Gevorkov,
- Thomas A. White,
- Aleksandra Tolstikova,
- Pablo Villanueva-Perez,
- Carolin Seuring,
- Steve Aplin,
- Armando D. Estillore,
- Jochen Küpper,
- Alexander Klyuev,
- Manuela Kuhn,
- Torsten Laurus,
- Heinz Graafsma,
- Diana C. F. Monteiro,
- Martin Trebbin,
- Filipe R. N. C. Maia,
- Francisco Cruz-Mazo,
- Alfonso M. Gañán-Calvo,
- Michael Heymann,
- Connie Darmanin,
- Brian Abbey,
- Marius Schmidt,
- Petra Fromme,
- Klaus Giewekemeyer,
- Marcin Sikorski,
- Rita Graceffa,
- Patrik Vagovic,
- Thomas Kluyver,
- Martin Bergemann,
- Hans Fangohr,
- Jolanta Sztuk-Dambietz,
- Steffen Hauf,
- Natascha Raab,
- Valerii Bondar,
- Adrian P. Mancuso,
- Henry Chapman,
- Anton Barty
Affiliations
- Oleksandr Yefanov
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Dominik Oberthür
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Richard Bean
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Max O. Wiedorn
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Juraj Knoska
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Gisel Pena
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Salah Awel
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Lars Gumprecht
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Martin Domaracky
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Iosifina Sarrou
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- P. Lourdu Xavier
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Markus Metz
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Saša Bajt
- Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Valerio Mariani
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Yaroslav Gevorkov
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Thomas A. White
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Aleksandra Tolstikova
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Pablo Villanueva-Perez
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Carolin Seuring
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Steve Aplin
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Armando D. Estillore
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Jochen Küpper
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Alexander Klyuev
- Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Manuela Kuhn
- Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Torsten Laurus
- Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Heinz Graafsma
- Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Diana C. F. Monteiro
- Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany
- Martin Trebbin
- Department of Chemistry, University at Buffalo, 359 Natural Sciences Complex, Buffalo, New York 14260, USA
- Filipe R. N. C. Maia
- Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden
- Francisco Cruz-Mazo
- Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092 Sevilla, Spain
- Alfonso M. Gañán-Calvo
- Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092 Sevilla, Spain
- Michael Heymann
- Intelligent Biointegrative Systems Group, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, D-70569 Stuttgart, Germany
- Connie Darmanin
- ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Sciences, La Trobe University, Victoria 3086, Australia
- Brian Abbey
- ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Sciences, La Trobe University, Victoria 3086, Australia
- Marius Schmidt
- Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA
- Petra Fromme
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- Klaus Giewekemeyer
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Marcin Sikorski
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Rita Graceffa
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Patrik Vagovic
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Thomas Kluyver
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Martin Bergemann
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Hans Fangohr
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Jolanta Sztuk-Dambietz
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Steffen Hauf
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Natascha Raab
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Valerii Bondar
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Adrian P. Mancuso
- European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany
- Henry Chapman
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- Anton Barty
- Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
- DOI
- https://doi.org/10.1063/1.5124387
- Journal volume & issue
-
Vol. 6,
no. 6
pp. 064702 – 064702-10
Abstract
The new European X-ray Free-Electron Laser (European XFEL) is the first X-ray free-electron laser capable of delivering intense X-ray pulses with a megahertz interpulse spacing in a wavelength range suitable for atomic resolution structure determination. An outstanding but crucial question is whether the use of a pulse repetition rate nearly four orders of magnitude higher than previously possible results in unwanted structural changes due to either radiation damage or systematic effects on data quality. Here, separate structures from the first and subsequent pulses in the European XFEL pulse train were determined, showing that there is essentially no difference between structures determined from different pulses under currently available operating conditions at the European XFEL.
