SCREENING OF WINTER SOFT WHEAT SAMPLES ON PRESENCE OF BROWN RUST RESISTANCE GENE LR17A

Abstract

The current paper considers the identification of the Lr17a brown rust resistance gene in the selection and collection samples of winter soft wheat developed by the FSBSI “Agricultural Research Center “Donskoy”. It has been determined that 49 lines out of the 717 studied genotypes possess a functional gene allele. A non-functional allele has been identified in 30 lines. 206 samples were identified with atypical alleles of the Lr17a gene. During the study there were used a CTAB method (a DNA isolation), PCR method (identification of resistance genes to the main winter wheat diseases) and an electrophoresis method on agarose gel. The purpose of the study was to screen collection and breeding material of winter soft wheat on presence of brown rust resistance gene Lr17a. As a result of the study, the identified samples with a functional allele of the Lr17a gene have been proposed to be used in breeding programs for pyramidation with other genes.

Keywords

• screening
• winter soft wheat
• gene
• resistance
• brown rust
• lr17a