THE DEVELOPMENT OF A PRODUCTION SCALE TECHNOLOGY OF RECOMBINANT FOLLICLE STIMULATING HORMONE BIOPROCESS IN CHO CELL LINE

Abstract

The article discusses the development of a production technique for recombinant follicle-stimulating hormone used in the treatment of infertility, in the culture of Chinese hamster ovary cells. As part of the creation of the technological platform receiving hormone the bioprocess in wave-induced motion bioreactor has been optimized. A number of parameters that influence on the bioprocess was studied, as follows: optimal cell density, concentration of metabolites, temperature shift and its duration.

Keywords

• bioprocess
• cell line
• upstream process
• follicle stimulating hormone