Inactivation of antibiotic resistant bacteria from stormwater runoff using UVA/LED and its potential risks

Abstract

Recently, increasing attention has been paid to antibiotic resistance in stormwater runoff. However, there is no available literature about the control of antibiotic resistant bacteria (ARB) through 365 nm ultraviolet light-emitting diode (UVA/LED). In this study, batch experiments were conducted to investigate ARB inactivation kinetics, effects of light intensity and water matrix (including suspended solid (SS) concentration, initial pH and bacteria concentration), and potential transmission risks after UVA/LED irradiation. Results showed that ARB inactivation efficiencies reached 6.31 log reduction at 8 mW/cm2 (86 J/cm2) of UVA/LED for 180 min. ARB inactivation efficiencies increased with the increase of light intensity, and showed a linear relationship. ARB inactivation decreased with increasing SS levels, and the largest inactivation efficiencies was 3.56 log reduction at 50 mg/L of SS. Initial pH had slight effect on ARB inactivation through UVA/LED irradiation. A low initial bacteria concentration (105 CFU/mL) was not necessarily associated with good ARB inactivation (3.59 log reduction). After UVA/LED irradiation, ARB was hardly detected during 12 hr of dark repair, and the transfer frequency of kanamycin resistance gene was increased to 5.43 × 10−4. These suggested that the application of UVA/LED to inactivate ARB in stormwater runoff was feasible and desirable in this study. HIGHLIGHTS ARB inactivation reached 6.31 log reduction at 8 mW/cm2 of UVA/LED for 180 min.; UVA/LED inactivation kinetics could be well described by the first-order model.; Initial pH and bacteria levels had weak effects for ARB inactivation.; Potential risks of ARB could be blocked after 60 min of UVA/LED disinfection.;

Keywords

• antibiotic resistant bacteria
• stormwater
• transmission
• uva/led disinfection