Phytochemical and Biological Studies of <i>Nepeta asterotricha</i> Rech. f. (Lamiaceae): Isolation of Nepetamoside

Abstract

The n-butanolic extract, from an Iranian specimen of Nepeta asterotricha Rech. f. (NABE), displayed anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated J774A.1 macrophages, which reduced nitrites and cytokines production. Bioassay guided fractionation of the extract led to the isolation of four iridoid glycosides, including a new one known as nepetamoside (1), one hexenyl-diglycoside, and some polyphenol and flavonoid components. None of the isolated iridoid components displayed significant effects on nitrites formation in an in vitro LPS-induced model of inflammation, thus suggesting that the plant anti-inflammatory effect is probably due to a synergistic action among its constituents.

Keywords

• <i>Nepeta asterotricha</i> Rech. f.
• Labiatae
• secondary metabolites
• iridoid glycosides
• nepetamoside
• NMR
• anti-inflammatory effect
• nitrites
• cytokines