Nature Communications (Oct 2016)
SUMOylated NKAP is essential for chromosome alignment by anchoring CENP-E to kinetochores
- Teng Li,
- Liang Chen,
- Juanxian Cheng,
- Jiang Dai,
- Yijiao Huang,
- Jian Zhang,
- Zhaoshan Liu,
- Ang Li,
- Na Li,
- Hongxia Wang,
- Xiaomin Yin,
- Kun He,
- Ming Yu,
- Tao Zhou,
- Xuemin Zhang,
- Qing Xia
Affiliations
- Teng Li
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Liang Chen
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Juanxian Cheng
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Jiang Dai
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Yijiao Huang
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Jian Zhang
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Zhaoshan Liu
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Ang Li
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Na Li
- Department of Gynecology, Affiliated Hospital of Academy of Military Medical Sciences
- Hongxia Wang
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Xiaomin Yin
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Kun He
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Ming Yu
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Tao Zhou
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Xuemin Zhang
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- Qing Xia
- State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis
- DOI
- https://doi.org/10.1038/ncomms12969
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 14
Abstract
The kinetochore-bound motor CENP-E plays a critical role in chromosome alignment. Here, the authors show that NF-κB activating protein (NKAP) dynamically localises to kinetochores, is SUMOylated during mitosis, and this modification is required for NKAP to bind CENP-E and localise CENP-E to the kinetochore.
