High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast

Elena Burlacu; Fredrik Lackmann; Lisbeth-Carolina Aguilar; Sergey Belikov; Rob van Nues; Christian Trahan; Ralph D. Hector; Nicholas Dominelli-Whiteley; Scott L. Cockroft; Lars Wieslander; Marlene Oeffinger; Sander Granneman

doi:10.1038/s41467-017-00761-8

Nature Communications (Sep 2017)

High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast

Elena Burlacu,
Fredrik Lackmann,
Lisbeth-Carolina Aguilar,
Sergey Belikov,
Rob van Nues,
Christian Trahan,
Ralph D. Hector,
Nicholas Dominelli-Whiteley,
Scott L. Cockroft,
Lars Wieslander,
Marlene Oeffinger,
Sander Granneman

Affiliations

Elena Burlacu: Centre for Synthetic and Systems Biology (SynthSys), University of Edinburgh
Fredrik Lackmann: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University
Lisbeth-Carolina Aguilar: Institut de Recherches Cliniques de Montréal RNP Biochemistry laboratory, Systems Biology Axis
Sergey Belikov: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University
Rob van Nues: Institute of Cell Biology, University of Edinburgh
Christian Trahan: Institut de Recherches Cliniques de Montréal RNP Biochemistry laboratory, Systems Biology Axis
Ralph D. Hector: Institute of Neuroscience and Psychology, University of Glasgow
Nicholas Dominelli-Whiteley: EaStCHEM School of Chemistry, University of Edinburgh
Scott L. Cockroft: EaStCHEM School of Chemistry, University of Edinburgh
Lars Wieslander: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University
Marlene Oeffinger: Institut de Recherches Cliniques de Montréal RNP Biochemistry laboratory, Systems Biology Axis
Sander Granneman: Centre for Synthetic and Systems Biology (SynthSys), University of Edinburgh

DOI: https://doi.org/10.1038/s41467-017-00761-8
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 14

Abstract

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Ribosome biogenesis is a dynamic process that involves the ordered assembly of ribosomal proteins and numerous RNA structural rearrangements. Here the authors apply ChemModSeq, a high-throughput RNA structure probing method, to quantitatively measure changes in RNA flexibility during the nucleolar stages of 60S assembly in yeast.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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