Nature Communications (May 2019)

qDSB-Seq is a general method for genome-wide quantification of DNA double-strand breaks using sequencing

  • Yingjie Zhu,
  • Anna Biernacka,
  • Benjamin Pardo,
  • Norbert Dojer,
  • Romain Forey,
  • Magdalena Skrzypczak,
  • Bernard Fongang,
  • Jules Nde,
  • Razie Yousefi,
  • Philippe Pasero,
  • Krzysztof Ginalski,
  • Maga Rowicka

DOI
https://doi.org/10.1038/s41467-019-10332-8
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 11

Abstract

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Measuring relative frequencies of DNA double-strand breaks between loci does not provide the full physiological relevance of those breaks. Here Rowicka and colleagues present qDSB-Seq method which uses spike-in double-strand breaks induced by a restriction enzyme to accurately quantify DNA damage.