Agarose Gel Analysis of 15–40-kb PCR Amplimers

Abstract

Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to reliably allow staining and photography. This paper describes a test system for Long PCR and demonstrates analysis of the PCR products on a gel run under standard low-voltage electrophoresis conditions.