Nature Communications (Feb 2020)

A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA

  • Olivier G. de Jong,
  • Daniel E. Murphy,
  • Imre Mäger,
  • Eduard Willms,
  • Antonio Garcia-Guerra,
  • Jerney J. Gitz-Francois,
  • Juliet Lefferts,
  • Dhanu Gupta,
  • Sander C. Steenbeek,
  • Jacco van Rheenen,
  • Samir El Andaloussi,
  • Raymond M. Schiffelers,
  • Matthew J. A. Wood,
  • Pieter Vader

DOI
https://doi.org/10.1038/s41467-020-14977-8
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 13

Abstract

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Extracellular vesicles (EV) facilitate intercellular transfer of biological material including RNA, but the regulatory mechanisms for their formation and transfer are incompletely known. Here the authors develop a CRISPR-based reporting system to detect the transfer of guide RNAs and identify genes not previously linked to EV-mediated RNA delivery.