A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA

Olivier G. de Jong; Daniel E. Murphy; Imre Mäger; Eduard Willms; Antonio Garcia-Guerra; Jerney J. Gitz-Francois; Juliet Lefferts; Dhanu Gupta; Sander C. Steenbeek; Jacco van Rheenen; Samir El Andaloussi; Raymond M. Schiffelers; Matthew J. A. Wood; Pieter Vader

doi:10.1038/s41467-020-14977-8

Nature Communications (Feb 2020)

A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA

Olivier G. de Jong,
Daniel E. Murphy,
Imre Mäger,
Eduard Willms,
Antonio Garcia-Guerra,
Jerney J. Gitz-Francois,
Juliet Lefferts,
Dhanu Gupta,
Sander C. Steenbeek,
Jacco van Rheenen,
Samir El Andaloussi,
Raymond M. Schiffelers,
Matthew J. A. Wood,
Pieter Vader

Affiliations

Olivier G. de Jong: Laboratory of Clinical Chemistry and Hematology, University Medical Center Utrecht
Daniel E. Murphy: Laboratory of Clinical Chemistry and Hematology, University Medical Center Utrecht
Imre Mäger: Department of Paediatrics, University of Oxford
Eduard Willms: Department of Paediatrics, University of Oxford
Antonio Garcia-Guerra: Department of Paediatrics, University of Oxford
Jerney J. Gitz-Francois: Laboratory of Clinical Chemistry and Hematology, University Medical Center Utrecht
Juliet Lefferts: Pediatric Pulmonology and Regenerative Medicine Center, University Medical Center Utrecht
Dhanu Gupta: Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet
Sander C. Steenbeek: Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute
Jacco van Rheenen: Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute
Samir El Andaloussi: Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet
Raymond M. Schiffelers: Laboratory of Clinical Chemistry and Hematology, University Medical Center Utrecht
Matthew J. A. Wood: Department of Paediatrics, University of Oxford
Pieter Vader: Laboratory of Clinical Chemistry and Hematology, University Medical Center Utrecht

DOI: https://doi.org/10.1038/s41467-020-14977-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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Extracellular vesicles (EV) facilitate intercellular transfer of biological material including RNA, but the regulatory mechanisms for their formation and transfer are incompletely known. Here the authors develop a CRISPR-based reporting system to detect the transfer of guide RNAs and identify genes not previously linked to EV-mediated RNA delivery.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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