Human pandemic K27-ST392 CTX-M-15 extended-spectrum β-lactamase-positive Klebsiella pneumoniae: A one health clone threatening companion animals
Luciano C.B.A. da Silva,
Brenda Cardoso,
Herrison Fontana,
Fernanda Esposito,
Silvia R.G. Cortopassi,
Fábio P. Sellera,
Nilton Lincopan
Affiliations
Luciano C.B.A. da Silva
Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil; School of Veterinary Medicine, Metropolitan University of Santos, Santos, Brazil
Brenda Cardoso
One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
Herrison Fontana
One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil; Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil
Fernanda Esposito
One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil; Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil
Silvia R.G. Cortopassi
Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
Fábio P. Sellera
School of Veterinary Medicine, Metropolitan University of Santos, Santos, Brazil; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil; Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil; Corresponding author at: School of Veterinary Medicine, Metropolitan University of Santos, Santos, Brazil.
Nilton Lincopan
One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Corresponding author at: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Extended spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae is a medically important pathogen that commonly causes human nosocomial infections. Since veterinary emergency and critical care services have also significantly progressed over the last decades, there are increasing reports of ESBL-producing K. pneumoniae causing hospital-associated infections in companion animals. We present microbiological and genomic analysis of a multidrug-resistant ESBL-positive K. pneumoniae (LCKp01) isolated from a fatal infection in a dog admitted to a veterinary intensive care unit. LCKp01 strain belonged to the sequence type ST392 and displays a KL27 (wzi-187) and O-locus 4 (O4). A broad resistome and presence of the blaCTX-M-15 ESBL gene were predicted. SNP-based phylogenomic analysis, using an international genome database, clustered LCKp01 (60–80 SNPs differences) with K. pneumoniae ST392 from human and animal infections, isolated at 4-year interval, whereas phylogeographical analysis confirmed successful expansion of ST392 as a global clone of One Health concern.
