Molecular Diagnosis of Human Metapneumovirus in Hospitalized Children with Acute Respiratory Tract Infections using RT-LAMP: A Population-Based Prospective Study

Abstract

Background: Human metapneumovirus (hMPV) is a major novel cause of acute respiratory infections ranging from wheezing to bronchiolitis and pneumonia in children worldwide. The aim of this study was to detect hMPV in hospitalized children with acute respiratory tract infections (ARTIs) by using reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay. Methods and Results: A total of 68 children with ARTIs who were clinically suspected of acquiring hMPV were included in the study in the period between January 2019 and February 2020. Posterior-pharyngeal (throat) swabs were obtained from each patient. hMPV RNA was revealed in 18(26.5%) cases. The age range was from <1 year to 10 years (mean age of 5.25±2.62). Sixteen (23.5%) of the participants were in the age group of <1 year, where the majority of hMPV-positive subjects (n=11) were found (16.2% of the total number of infected children) (P=0.0025). The majority of hMPV-negative subjects (n=15) were found in the age group of 5-10 years (22% of the total number of infected children) (P=0.0025). Cough, fever, and shortness of breath were common symptoms in hMPV-positive children: 15(83.3%), 13(72.2%), and 12(66.7%), respectively. There was a statistically significant correlation between common clinical symptoms and the age group of hMPV-positive children: symptoms were common in the age group of <1 year. Conclusion: Our study represents the first report in Khartoum, Sudan, on the detection of hMPV using RT-LAMP. RT-LAMP is a valuable, quick diagnostic technique for hMPV detection.

Keywords

• human metapneumovirus
• acute respiratory tract infections
• rt-lamp