Microorganisms (Apr 2024)

<i>Pannonibacter anstelovis</i> sp. nov. Isolated from Two Cases of Bloodstream Infections in Paediatric Patients

  • Stefano Castellana,
  • Vittoriana De Laurentiis,
  • Angelica Bianco,
  • Laura Del Sambro,
  • Massimo Grassi,
  • Francesco De Leonardis,
  • Anna Maria Derobertis,
  • Carmen De Carlo,
  • Eleonora Sparapano,
  • Adriana Mosca,
  • Stefania Stolfa,
  • Luigi Ronga,
  • Luigi Santacroce,
  • Maria Chironna,
  • Michela Parisi,
  • Loredana Capozzi,
  • Antonio Parisi

DOI
https://doi.org/10.3390/microorganisms12040799
Journal volume & issue
Vol. 12, no. 4
p. 799

Abstract

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This study describes two cases of bacteraemia sustained by a new putative Pannonibacter species isolated at the U.O.C. of Microbiology and Virology of the Policlinico of Bari (Bari, Italy) from the blood cultures of two patients admitted to the Paediatric Oncohaematology Unit. Pannonibacter spp. is an environmental Gram-negative bacterium not commonly associated with nosocomial infections. Species identification was performed using Sanger sequencing of the 16S rRNA gene and Whole-Genome Sequencing (WGS) for both strains. Genomic analyses for the two isolates, BLAST similarity search, and phylogeny for the 16S rDNA sequences lead to an assignment to the species Pannonibacter phragmitetus. However, by performing ANIb, ANIm, tetranucleotide correlation, and DNA-DNA digital hybridization, analyses of the two draft genomes showed that they were very different from those of the species P. phragmitetus. MALDI-TOF analysis, assessment of antimicrobial susceptibility by E-test method, and Analytical Profile Index (API) tests were also performed. This result highlights how environmental bacterial species can easily adapt to the human host and, especially in nosocomial environments, also gain pathogenic potential through antimicrobial resistance.

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