Nature Communications (Aug 2021)

Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes

  • Alex S Genshaft,
  • Carly G. K. Ziegler,
  • Constantine N. Tzouanas,
  • Benjamin E. Mead,
  • Alex M. Jaeger,
  • Andrew W. Navia,
  • Ryan P. King,
  • Miyeko D. Mana,
  • Siyi Huang,
  • Vanessa Mitsialis,
  • Scott B. Snapper,
  • Ömer H. Yilmaz,
  • Tyler Jacks,
  • Jeffrey F. Van Humbeck,
  • Alex K. Shalek

DOI
https://doi.org/10.1038/s41467-021-25279-y
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

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Spatial transcriptomics aims to pair omic data with tissue structure. Here the authors report Spatially PhotoActivatable Colour Encoded Cell Address Tags (SPACECAT) to track and isolate live cells by location; this enables spatially informed downstream assays like scRNA-seq and flow cytometry.