Nature Communications (Feb 2023)
Direct digital sensing of protein biomarkers in solution
- Georg Krainer,
- Kadi L. Saar,
- William E. Arter,
- Timothy J. Welsh,
- Magdalena A. Czekalska,
- Raphaël P. B. Jacquat,
- Quentin Peter,
- Walther C. Traberg,
- Arvind Pujari,
- Akhila K. Jayaram,
- Pavankumar Challa,
- Christopher G. Taylor,
- Lize-Mari van der Linden,
- Titus Franzmann,
- Roisin M. Owens,
- Simon Alberti,
- David Klenerman,
- Tuomas P. J. Knowles
Affiliations
- Georg Krainer
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Kadi L. Saar
- Yusuf Hamied Department of Chemistry, University of Cambridge
- William E. Arter
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Timothy J. Welsh
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Magdalena A. Czekalska
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Raphaël P. B. Jacquat
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Quentin Peter
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Walther C. Traberg
- Department of Chemical Engineering and Biotechnology, University of Cambridge
- Arvind Pujari
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Akhila K. Jayaram
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Pavankumar Challa
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Christopher G. Taylor
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Lize-Mari van der Linden
- Biotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden
- Titus Franzmann
- Biotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden
- Roisin M. Owens
- Department of Chemical Engineering and Biotechnology, University of Cambridge
- Simon Alberti
- Biotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden
- David Klenerman
- Yusuf Hamied Department of Chemistry, University of Cambridge
- Tuomas P. J. Knowles
- Yusuf Hamied Department of Chemistry, University of Cambridge
- DOI
- https://doi.org/10.1038/s41467-023-35792-x
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 21
Abstract
There are limitations with current protein sensing methods. Here the authors report DigitISA, a digital immunosensor assay based on microchip electrophoretic separation and single-molecule detection that enables quantitation of protein biomarkers in a single, solution-phase step.
