DNA-Interactive and Damage Study with <i>meso</i>-Tetra(2-thienyl)porphyrins Coordinated with Polypyridyl Pd(II) and Pt(II) Complexes
Bernardo Almeida Iglesias,
Níckolas Pippi Peranzoni,
Sophia Iwersen Faria,
Luana Belo Trentin,
André Passaglia Schuch,
Otávio Augusto Chaves,
Renan Ribeiro Bertoloni,
Sofia Nikolaou,
Kleber Thiago de Oliveira
Affiliations
Bernardo Almeida Iglesias
Bioinorganic and Porphyrinoids Materials Laboratory, Department of Chemistry, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil
Níckolas Pippi Peranzoni
Laboratory of Photobiology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil
Sophia Iwersen Faria
Laboratory of Photobiology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil
Luana Belo Trentin
Laboratory of Photobiology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil
André Passaglia Schuch
Laboratory of Photobiology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil
Otávio Augusto Chaves
CQC-IMS, Department of Chemistry, University of Coimbra, Rua Larga, 3004-535 Coimbra, Portugal
Renan Ribeiro Bertoloni
Laboratory of Biological Activity and Supramolecular Chemical of Coordination Compounds (LABiQSC2), Department of Chemistry, FFCLRP, University of São Paulo, Ribeirão Preto 14040-901, SP, Brazil
Sofia Nikolaou
Laboratory of Biological Activity and Supramolecular Chemical of Coordination Compounds (LABiQSC2), Department of Chemistry, FFCLRP, University of São Paulo, Ribeirão Preto 14040-901, SP, Brazil
Kleber Thiago de Oliveira
Department of Chemistry, Federal University of São Carlos, Rod. Washington Luiz, km 235, São Carlos 13565-905, SP, Brazil
We report the DNA-binding properties of three porphyrins with peripheral thienyl substituents (TThPor, PdTThPor and PtTThPor). The binding capacity of each porphyrin with DNA was determined by UV-Vis and steady-state fluorescence emission spectroscopy combined with molecular docking calculations. The results suggest that the interaction of these compounds probably occurs via secondary interactions via external grooves (minor grooves) around the DNA macromolecule. Moreover, porphyrins containing peripheral Pd(II) or Pt(II) complexes (PdTThPor and PtTThPor) were able to promote photo-damage in the DNA.
