Oxidative stress in hyperthermia

Abstract

Oxidative stress is a risk factor for disease development and cardiovascular system, diabetes, neurodegenerative diseases, etc. The resulting product is thus free radical lipid peroxidation - malondialdehyde (MDA) is atherogenic modification of low density lipoproteins (LDL), so that they acquire the ability to be rapidly accumulated in the cells of the vessel walls, causing pre-aterosklerotic lipoid damages. The aim of this study was to investigate the effect of high temperature (one of the environmental factors) for key operating parameters: the content of secondary products of free radical oxidation polyene lipids - mDa in blood plasma and erythrocyte antioxidant activity of key enzymes. Hyperthermia was triggered in practically healthy volunteers (6 males, 22-46years). The analysis was made using a recording spectrophotometer Hitachi-557 (Japan). The statistical analysis was performed using the non-parametric Wilcoxon-test (Attestat program). MDA levels in the blood plasma, the activity of key antioxidant enzymes in erythrocytes were determined through divided intervals in study participants. A significant increase in MDA content (62 %) and reduction of catalase activity (11 %) and glutathione peroxidase (19 %) were revealed after 30 days in the conditions of hyperthermia test.

Keywords

• reactive oxygen species
• free radical oxidation
• malondialdehyde
• catalase
• glutathione peroxidase