Circ_0032821 is involved in sorafenib's inhibition of the proliferation, migration and invasion of breast cancer cell line T47D

Abstract

Objective To investigate the effect and possible mechanism of sorafenib on the proliferation, migration and invasion of breast cancer cell line T47D. Methods Effects of sorafenib (2.5, 5 and 10 μmol/L) on T47D cells proliferation, colony formation, migration and invasion were detected by CCK-8 experiment, plate cloning experiment, scratch healing experiment and Transwell experiment. RT-qPCR was applied to detect circ_0032821 expression. The circ_0032821 small interfering RNA(si-circ_0032821) and circ_0032821 over-expression plasmid were transfected into T47D cells respectively. And the effect of interference with circ_0032821 or circ_0032821 over-expression combined with sorafenib on the malignant behavior of T47D cells was detected. Results The cell proliferation inhibition rate T47D cells were significantly increased after sorafenib treatment (P＜0.05); The colony formation number, scratch healing rate, invasion number, and circ_0032821 expression were significantly decreased(P＜0.05).After silencing with the expression of circ_0032821, the proliferation inhibition rate of T47D cells was significantly increased (P＜0.05); The colony formation number, scratch healing rate and invasion number were significantly reduced(P＜0.05). Circ_0032821 over-expression significantly reduced the effects of sorafenib on the proliferation, migration colony formation and invasion of T47D cells (P＜0.05). Conclusions Sorafenib can inhibit T47D cell proliferation, migration and invasion. And the mechanism may be explained by the down-regulation of circ_0032821.

Keywords

• sorafenib| breast cancer| proliferation| migration| circ_0032821