HPLC-DAD-Guided Isolation of Diversified Chaetoglobosins from the Coral-Associated Fungus <i>Chaetomium globosum</i> C2F17
Xiao-Wei Luo,
Cheng-Hai Gao,
Hu-Mu Lu,
Jia-Min Wang,
Zi-Qi Su,
Hua-Ming Tao,
Xue-Feng Zhou,
Bin Yang,
Yong-Hong Liu
Affiliations
Xiao-Wei Luo
Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China
Cheng-Hai Gao
Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China
Hu-Mu Lu
Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China
Jia-Min Wang
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Zi-Qi Su
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515 China
Hua-Ming Tao
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515 China
Xue-Feng Zhou
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Bin Yang
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Yong-Hong Liu
Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China
Cytochalasans have continuously aroused considerable attention among the chemistry and pharmacology communities due to their structural complexities and pharmacological significances. Sixteen structurally diverse chaetoglobosins, 10-(indol-3-yl)-[13]cytochalasans, including a new one, 6-O-methyl-chaetoglobosin Q (1), were isolated from the coral-associated fungus Chaetomium globosum C2F17. Their structures were accomplished by extensive spectroscopic analysis combined with single-crystal X-ray crystallography and ECD calculations. Meanwhile, the structures and absolute configurations of the previously reported compounds 6, 12, and 13 were confirmed by single-crystal X-ray analysis for the first time. Chaetoglobosins E (6) and Fex (11) showed significant cytotoxicity against a panel of cancer cell lines, K562, A549, Huh7, H1975, MCF-7, U937, BGC823, HL60, Hela, and MOLT-4, with the IC50 values ranging from 1.4 μM to 9.2 μM.
