Nature Communications (Nov 2021)

Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons

  • Diogo Bessa-Neto,
  • Gerti Beliu,
  • Alexander Kuhlemann,
  • Valeria Pecoraro,
  • Sören Doose,
  • Natacha Retailleau,
  • Nicolas Chevrier,
  • David Perrais,
  • Markus Sauer,
  • Daniel Choquet

DOI
https://doi.org/10.1038/s41467-021-27025-w
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 16

Abstract

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Visualisation of TARP localisation is hindered by existing imaging tools. Here the authors report a labelling and imaging platform using genetic code expansion and non-canonical amino acids; they use this to fluorescently label live neurons and localise TARP proteins using super resolution microscopy.