Protective Effects of the Hydroethanolic Extract of <i>Fridericia chica</i> on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)
Neda Alvarez-Ortega,
Karina Caballero-Gallardo,
María Taboada-Alquerque,
Jackeline Franco,
Elena E. Stashenko,
Cristina Juan,
Ana Juan-García,
Jesus Olivero-Verbel
Affiliations
Neda Alvarez-Ortega
Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130014, Colombia
Karina Caballero-Gallardo
Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130014, Colombia
María Taboada-Alquerque
Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130014, Colombia
Jackeline Franco
Metabolite Profiling Facility, Bindley Bioscience Center, Purdue University, 1203 W State St., West Lafayette, IN 47907, USA
Elena E. Stashenko
Center for Chromatography and Mass Spectrometry CROM-MASS, Research Center for Biomolecules CIBIMOL, School of Chemistry, Universidad Industrial de Santander, Bucaramanga 680006, Colombia
Cristina Juan
Facultat de Farmàcia—Avda, Universitat de València, Vicent Andrés Estellés, s/n, 46100 València, Spain
Ana Juan-García
Facultat de Farmàcia—Avda, Universitat de València, Vicent Andrés Estellés, s/n, 46100 València, Spain
Jesus Olivero-Verbel
Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130014, Colombia
Fridericia chica (Bignoniaceae) is a traditional medicinal plant. The aim of this research was to determine the protective effects of the hydroethanolic extract from the F. chica leaves (HEFc) against the cytotoxicity of zearalenone (α-ZEL) and β-ZEL on SH-SY5Y cells. Free radical scavenging activity of HEFc was evaluated using the DPPH method. The cytotoxicity of both zearalenone metabolites and HEFc was examined using MTT test, as was the cytoprotective effects of the HEFc on cells treated with these mycotoxins. The chemical composition of HEFc was determined using UPLC-QTOF-MS/MS. HEFc elicited good DPPH radical scavenging activity following a concentration-dependent relationship. Cells exposed to α-ZEL exhibited a viability ˂50% after 48 h of treatment (25 and 50 µM), while those exposed to β-ZEL showed viability ˂50% (100 µM) and ˂25% (25-100 µM) after 24 and 48 h of exposure, respectively. HEFc showed a significant increase in cell viability after exposure to α-ZEL (25 and 50 µM) and β-ZEL (6–100 µM) (p F. chica grown in Colombian Caribbean can protect against the effects of mycotoxins and it is a valuable source of compounds with antioxidant properties.
