The anatomical and histological structures of buzzard"s (buteo buteo) small intestine and liver, and immunohistochemical localization of catalase

Abstract

This study aimed to examine the structure of the buzzard"s Buteo buteo) small intestine and liver, and the localization of catalase in those organs. Crossman"s modified triple stain and Periodic Acid Schiff (PAS) stain were applied to the samples for histological examination. To detect catalase localization in the tissues, the avidin-biotin-peroxidase complex (ABC) technique was used. It was observed that the general histological structure of the buzzard"s small intestine and liver was similar to that of other avian species. Catalase immunoreactivity in the lamina propria and submucosa in the small intestine were also observed, as well as catalase immunoreactivity in the hepatocyte cytoplasm in the liver. Some areas in the small intestine and liver had catalase immunoreactivity, which means that an antioxidant defence occurs in those areas.

Keywords

• antioxidant enzyme
• buzzard
• catalase
• immunohistochemistry
• liver
• small intestine