Identification and phylogenetic positioning of salmonella dublin from aborted cattle materials

Abstract

This study was aimed to isolate, and characterize the Salmonella Dublin in the liver, lung tissues and the abomasum contents of 367 aborted cattle fetal samples obtained from four different cities (Agri, Erzincan, Erzurum and Kars) of Turkey by using molecular methods. After proper incubation of the tissues and contents, Salmonella spp. identification was performed. Colonies with suspicion of Salmonella were boiled on a dry heat block for DNA extraction, while multiplex PCR was used for species identification of Salmonella spp. and identification of S. Dublin. The multiplex PCR, 16S rDNA sequence, and phylogenetic analysis were performed on an isolate with a band profile specific to S. Dublin. The S. Dublin identification was performed according to BLAST analysis and similarity scores obtained from NCBI GenBank. Molecular prevalence of S. Dublin in the aborted fetal samples of cattle was 0.82%. The 16S rRNA sequence results of the isolate was found to be similar to many S. Dublin strains. The partial sequence of the 16S rRNA gene region of the isolate was recorded in the GenBank database with the name strain Erzurum VCRI and access number MZ452230. In conclusion, S. Dublin was identified in the aborted cattle fetuses and phylogenetic position was determined for S. Dublin using the sequence analysis of 16S rDNA gene region.

Keywords

• cattle
• salmonella dublin
• phylogenetic
• sequence