Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles
Chenxing Guo,
Jianfang Wang,
Xianzhe Chen,
Yujiao Li,
Lifang Wu,
Jin Zhang,
Cheng-an Tao
Affiliations
Chenxing Guo
Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, China
Jianfang Wang
College of Liberal Arts and Science, National University of Defense Technology, Changsha 410073, China
Xianzhe Chen
College of Liberal Arts and Science, National University of Defense Technology, Changsha 410073, China
Yujiao Li
College of Liberal Arts and Science, National University of Defense Technology, Changsha 410073, China
Lifang Wu
Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, China
Jin Zhang
Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, China
Cheng-an Tao
College of Liberal Arts and Science, National University of Defense Technology, Changsha 410073, China
A biosensor based on a combination of cytochrome c (Cyt c), electrochemical reduced graphene oxides (ERGO), and gold nanoparticles (AuNPs) on a glassy carbon electrode (GCE) was fabricated. The proposed biosensor electrode was denoted as GCE/ERGO-Nafion/AuNPs/Cyt c/Nafion, where ERGO-Nafion was deposited by dropping graphene oxides-Nafion mixed droplet first and following electrochemical reduction, AuNPs were directly deposited on the surface of the ERGO-Nafion modified electrode by electrochemical reduction, and other components were deposited by the dropping-dry method. The effect of the deposition amount of AuNPs on direct electrochemistry of Cyt c in the proposed electrode was investigated. The hydrogen peroxide was taken to evaluate the performance of the proposed biosensor. The results showed that the biosensor has great analytical performance, including a high sensitivity, a wide linear range, a low detection limit, and good stability, reproducibility, and reliability.
