LncRNA-mRNA Expression Profiles of Osteoclast After Conditional Knockout HIF-1α

Yuanye Tian; Yuanye Tian; Qi Shao; Qi Shao; Jiahong Gu; Jiahong Gu; Yi Tang; Yi Tang; Miaomiao Bie; Miaomiao Bie; Yangyifan Zhou; Yangyifan Zhou; Chunan Cheng; Chunan Cheng; Yi Liang; Yi Liang; Qian Zhang; Qian Zhang; Feiwu Kang; Feiwu Kang

doi:10.3389/fgene.2022.909095

Frontiers in Genetics (Jun 2022)

LncRNA-mRNA Expression Profiles of Osteoclast After Conditional Knockout HIF-1α

Yuanye Tian,
Yuanye Tian,
Qi Shao,
Qi Shao,
Jiahong Gu,
Jiahong Gu,
Yi Tang,
Yi Tang,
Miaomiao Bie,
Miaomiao Bie,
Yangyifan Zhou,
Yangyifan Zhou,
Chunan Cheng,
Chunan Cheng,
Yi Liang,
Yi Liang,
Qian Zhang,
Qian Zhang,
Feiwu Kang,
Feiwu Kang

Affiliations

Yuanye Tian: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Yuanye Tian: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China
Qi Shao: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Qi Shao: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China
Jiahong Gu: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Jiahong Gu: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China
Yi Tang: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Yi Tang: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China
Miaomiao Bie: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Miaomiao Bie: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China
Yangyifan Zhou: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Yangyifan Zhou: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China
Chunan Cheng: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Chunan Cheng: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China
Yi Liang: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Yi Liang: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China
Qian Zhang: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Qian Zhang: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China
Feiwu Kang: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China
Feiwu Kang: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China

DOI: https://doi.org/10.3389/fgene.2022.909095
Journal volume & issue: Vol. 13

Abstract

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Background: Osteoclasts, which are multinucleated cells formed by monocyte fusion, play a key role in bone resorption. Hypoxia-inducible factor (HIF)-1α is vital for the development of osteoclasts in hypoxic environments and during bone resorption. However, additional research is required to further study the HIF-1α-dependent regulation of osteoclast differentiation at the genetic level.Methods: In our study, RNA sequencing (RNA-seq) was used to identify the expression profiles of long noncoding RNAs (lncRNAs) and mRNAs in conditional HIF-1α-knockout osteoclasts.Results: A total of 1,320 mRNAs and 95 lncRNAs were differentially expressed. The expression of lncRNAs MSTRG.7566.12 and MSTRG.31769.2 were strongly negatively correlated with that of Mmp9, Ctsk, etc.Conclusion: Our research provides a basis for further understanding the role of mRNAs and lncRNAs in conditional HIF-1α-knockout osteoclasts, and many of these molecules may be potential targets for treating bone diseases related to HIF-1α.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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