A new test for autism spectrum disorder: Metabolic data from different cell types
Sujata Srikanth,
Lauren Cascio,
Rini Pauly,
Kelly Jones,
Skylar Sorrow,
Rossana Cubillan,
Chin-Fu Chen,
Cindy D. Skinner,
Kevin Champaigne,
Roger E. Stevenson,
Charles E. Schwartz,
Luigi Boccuto
Affiliations
Sujata Srikanth
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Lauren Cascio
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Rini Pauly
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Kelly Jones
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Skylar Sorrow
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Rossana Cubillan
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Chin-Fu Chen
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Cindy D. Skinner
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Kevin Champaigne
Circa Bioscience, Greenwood, 113 Gregor Mendel Circle, Greenwood, SC 29646, USA
Roger E. Stevenson
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Charles E. Schwartz
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA
Luigi Boccuto
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA; School of Nursing, College of Behavioral, Social and Health Sciences, Clemson University, 508 Edwards Hall, Clemson, SC 29634, USA; Corresponding author at: School of Nursing, College of Behavioral, Social and Health Sciences, Clemson University, 508 Edwards Hall, Clemson, SC 29634, USA.
Experiments employing the Phenotype Mammalian Microarray (PM-M) technology were performed on lymphoblastoid cell lines (LCLs) from individuals with autism spectrum disorder (ASD) and age-matched controls. We used the custom-made PM-M plate designed to assess differential utilization of the amino acid tryptophan. Multiple parameters such as the sample size, incubation time, and cell concentration have been tested, leading to optimized protocols and minimized background noise by variable selection while controlling for false discoveries. The assay generated data based on the production of nicotinamide adenine dinucleotide (NADH) in the presence of different compounds containing tryptophan and showed clear differences between ASD and control samples.
