Data on hypoxia-induced VEGF, leptin and NF-kB p65 expression
Azizah Al-Anazi,
Ranjit Parhar,
Soad Saleh,
Reem Al-Hijailan,
Angela Inglis,
Mansour Al-Jufan,
Mohammed Bazzi,
Sarwar Hashmi,
Walter Conca,
Kate Collison,
Futwan Al-Mohanna
Affiliations
Azizah Al-Anazi
Department of Cell Biology, Riyadh 11211, Saudi Arabia
Ranjit Parhar
Department of Cell Biology, Riyadh 11211, Saudi Arabia
Soad Saleh
Department of Cell Biology, Riyadh 11211, Saudi Arabia
Reem Al-Hijailan
Department of Cell Biology, Riyadh 11211, Saudi Arabia
Angela Inglis
Department of Cell Biology, Riyadh 11211, Saudi Arabia
Mansour Al-Jufan
Heart Centre, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia
Mohammed Bazzi
Department of Biochemistry, College of Science, King Saud University, Riyadh 12372, Saudi Arabia
Sarwar Hashmi
Developmental Biology, Center for Vector Biology, Rutgers University, New Brunswick, NJ 08901, United States
Walter Conca
Department of Cell Biology, Riyadh 11211, Saudi Arabia; Department of Medicine, King Faisal Specialist Hospital and Research Centre, Alfaisal University, Riyadh 11211, Saudi Arabia; College of Medicine, Alfaisal University, Riyadh 11211, Saudi Arabia
Kate Collison
Department of Cell Biology, Riyadh 11211, Saudi Arabia
Futwan Al-Mohanna
Department of Cell Biology, Riyadh 11211, Saudi Arabia; College of Medicine, Alfaisal University, Riyadh 11211, Saudi Arabia; Correspondence to: Department of Cell Biology, MBC 03, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia.
The data set presented here is associated with the article “Intracellular calcium and NF-kB regulate hypoxia-induced leptin, VEGF, IL-6 and adiponectin secretion in human adipocytes” (Al-Anazi et al., 2018). Data illustrate hypoxia-induced VEGF and leptin expression in human adipocytes treated with the calcium chelator BAPTA-AM (1 µM). It also shows NF-κB p65 induced expression by hypoxia. Preadipocytes were differentiated for 14 days and then subjected to 0.5–1.5% oxygen in the presence and absence of BAPTA-AM or the NF-κB inhibitor SN50 for 48 h prior to RNA isolation and PCR analysis.
