Genome Sequence Resource of Burkholderia gladioli KRS027: A Strain with Antifungal Activity Against Plant Pathogens

Abstract

Some species of Burkholderia have been studied extensively for their ability to control plant diseases and promote plant growth. Burkholderia gladioli KRS027 is a strain isolated from the rhizosphere soil of a healthy plant in a pathogen-infested cotton field in Xinjiang, China, which had an inhibitory ability against various plant-pathogenic fungi. To understand its biocontrol mechanism, we assembled a high-quality genome of KRS027 using the PacBio-HiFiReads. The KRS027 genome contained two circular DNA chromosomes and one plasmid, of which Chr1 contained 4,187,756 bp with 67.95% GC content, Chr2 had 3,892,275 bp with 68.56% GC content, and the plasmid comprised 177,327 bp with 61.11% GC content. Moreover, the genome contains 20 secondary metabolite biosynthesis gene clusters involving polyketides (PKS), nonribosomal peptides (NRPS), hybrid NRPS/PKS, and terpene. The genes involved in biosynthesis of phytotoxins, such as toxoflavin, rhizotoxin, and bongkrekic acid, were not detected. The high-quality genome resource of strain KRS027 will provide a molecular basis for further research on its biocontrol mechanisms. [Figure: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Keywords

• biocontrol
• Burkholderia gladioli
• genome
• plant pathogen