Conversion of Deoxynivalenol to 3-acetyl deoxynivalenol in wheat and tobacco through the expression of Synthetic Acetyltransferase gene

Abstract

Detoxification of deoxynivalenol (DON) is one of the molecular mechanisms of resistance to Fusarium Head Blight desease in wheat, which causes by Fusarium graminearum. Mycotoxin producing Fusarium spices have some enzymes to reduce toxic effects. They can change trichothecenes to less toxic compounds by using trichothecene 3-O-acetyletransferase (Tri101) enzyme. This enzyme converts trichothecene to less toxic compounds by replaceing OH group on C3 with acetyl group. It had shown that a yeast acetyltransferase, AYT1, can convert DON to 3A DON and reduce it toxicity. In this study synthetic AYT1 gene was used for tobacco transformation and transiently expressed in wheat plants. The results showed that the synthetic AYT1 expresses in tobacco as well as wheat. Acetyltransferase activity analyses by thin layer chromatography confirmed that this enzyme can convert DON to 3-A DON that leads to reduce poisonous effect of mycotoxin.

Keywords

• acetyltransferase. deoxynivalenol .fusarium head blight. detoxification