Eco-friendly synthesis of silver nanoparticles by Bacillus subtilis and their antibacterial activity

Abstract

Bacillus subtilis was used for biogenic of silver nanoparticles. Characterization of the prepared silver nanoparticles was done by UV–Vis spectroscopy, Transmission Electron Microscopy (TEM), and Fourier Transform Infrared Spectroscopy (FT-IR). The particle size of the prepared nanoparticles ranges from 3 to 20 nm with spherical or roughly spherical forms. The antimicrobial efficacy of the produced nanoparticles was investigated against five strains of multidrug resistant microorganisms including: Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Klebsiella. pneumoniae, Escherichia coli and Candida albicans tested as yeast. During this study, the minimum inhibitory concentrations (MICs) and the minimum lethal concentrations (MLCs) of synthesized silver nanoparticles were detected using selected strains of the genus Bacillus by a broth dilution method. The rate of MIC of the prepared silver nano-particles versus the investigated clinical isolates exhibit a massive anti-microbial efficacy; (230 µgml−1) for MRSA; 180 for Staphylococcus epidermidis, 200 for Escherichia coli and 100 µgml−1 for Candida albicans. On the other hand, the lowest anti-microbial efficacy (300 µgml−1) was appeared for Klebsiella pneumonia. The obtained results demonstrated the effectiveness of the biogenic nanoparticles and the possibility of using them as a new method in combating infectious diseases.

Keywords

• Bacillus subtilis
• AgNPs
• Antimicrobial activity
• Green biosynthesis